DWR Diagnostics

Cytology and Dermatology

Cytology

Test name

Test details

Sample requirement

Test code

Turnaround time

FNA

Fine needle aspirate of one site (unlimited number of slides)

Glass slides

FNA

Same day

Extra site(s)

Glass slides

FNA2, 3 etc.

*NB: if samples are non-diagnostic, repeat FNAs are free of charge (specify 1st lab ref number, submit within 1 month) FNAs from multiple lymph nodes are usually charged as one site, unless there are different pathological processes present

BAL

Bronchoalveolar lavage cytology.

If multiple sites/tubes are submitted additional samples are charged at £9 per sample. Please indicate if you would like the samples pooled.

EDTA, slides +

plain sterile tube

BAL

Same day Culture + 4 days

With culture including enrichment culture and selective medium for Bordatella

BALC

MYCP

Culture + 4 days

4 days

CSF

Cytology, total protein, nucleated cell count, red cell count

2 EDTA samples, 1 fixed with one drop of serum (label fixed)

CSF

Same day

+ Bacterial culture

Culture performed following enrichment to maximise yield

Plain tube

CSFC

+ 4 days

Synovial fluid

Cytology, total protein, WBC count, RBC count

EDTA, slides

SFA

Same day

Above evaluation of 3 joints in polyarthropathy

EDTA, slides

SFPA

Cytology, protein, cell counts and culture/ sensitivity of 1 joint including enrichment culture to maximise yield

+ blood culture medium or enrichment broth

SFAC

Culture + up to 12 days

Synovial fluid smears only (1 joint)

Slides

SFC

Same day

Each additional joint

Slides

Effusion

Includes pleural, peritoneal and pericardial fluids. Cytology, total protein, albumin, globulin, nucleated cell count, red cell count

EDTA

EFF

Same day

With culture including enrichment culture

+ plain, sterile tube

EFFC

+ 3-5 days

Suspected FIP effusion

Cytology, total protein, albumin, globulin, nucleated cell count, red cell count and Coronavirus PCR

Fluid in plain + EDTA

FIPC

3 days

Chylous effusion

Cytology, total protein, WBC count, RBC count, triglyceride, cholesterol, C:T ratio

EDTA+ heparin

CHYL

Same day

Miscellaneous fluid (e.g. from a cyst/mass)

Cytology of cytospin

EDTA fluid

MF

Same day

With culture including enrichment culture

+ plain tube

MFC

+ 4 days

Prostatic wash

Cytology of cytospin

EDTA

PW

Same day

With culture including enrichment culture

+ plain sterile pot

PWC

+ 3 days

Bile cytology

Cytology of cytospin

Bile

BICY

Same day

Urine cytology

Cytology of stained sediment (used in suspected neoplasia)

Plain urine + EDTA

UCYT

Same day

Gram stain

For FNA’s or fluid samples to guide antibiotic therapy pending culture

Glass slides, fluid

GS

Same day

ZN Stain

To identify acid fast organisms including mycobacteria

Glass slides, fluid

ZN

Next day

Flow cytometry

Immunophenotyping of lymphoma using a FNA harvested into culture medium, includes cytology

Glass slides & aspirates in saline/plasma (see test protocols)

FLOL

Same day or next day

Immuno- cytochemistry

Antibody staining of cytology smears for classification of tumours

FNA on lysine coated slides (contact lab)

ICC

Up to 7 days

Dermatology

Test name

Test details

Sample requirement

Test code

Turnaround time

Dermatophyte culture

To identify ringworm

Hair or coat brushing

DERC

Up to 21 days

Skin scrape, sellotape slide and trichogam

Microscopy to identify parasites, bacteria, yeasts, dermatophyte spores, hair defects

Skin scrape, sellotape slides, hair

SSS

Same day

Dermatophyte culture + skin scrape/trichogram

To identify hair defects, ectoparasites, yeasts and dermatophytosis

Hair or coat brushing, skin scrape, sellotape slides

DETR

Dermatophyte culture up to 21 days

Ear cytology

To identify bacteria and malassezia

Cotton swab rolled onto slide

EARC

Same day

Sarcoptic mange antibodies

Antibodies to detect sarcoptic mange

Serum

SMAN

Up to 7 days

Sellotape slide function: Searching for superficial bacteria

Skin scrap function: Searching for ectoparasites. Suggest scraping the skin with a blunt scalpel blade until there is capillary ooze, and then smearing the harvested material on a glass slide. A drop of liquid paraffin may facilitate the collection of scraped material. Squeezing the skin between thumb and finger whilst scraping may extrude material from deeper in the hair follicles.

Test and Profiles

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